PoplarV1, Main, Exploration, bibRecord, 001589

[Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera].

Identifieur interne : 001589 ( Main/Exploration ); précédent : 001588; suivant : 001590

[Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera].

Auteurs : Jun Wu ; Jun-Hong Zhang ; Meng-Hui Huang ; Min-Hui Zhu ; Zai-Kang Tong

Source :

Yi chuan = Hereditas [ 0253-9772 ] ; 2016.

RBID : pubmed:26907779

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Betula (genetics), Cloning, Molecular (MeSH), Gene Expression Profiling (methods), Gene Expression Regulation, Plant (MeSH), MicroRNAs (genetics), Molecular Sequence Data (MeSH), Nitrates (metabolism), Plant Proteins (genetics), RNA, Plant (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Time Factors (MeSH).
MESH :
- chemical , genetics : MicroRNAs, Plant Proteins, RNA, Plant.
- genetics : Betula.
- chemical , metabolism : Nitrates.
- methods : Gene Expression Profiling.
- Amino Acid Sequence, Cloning, Molecular, Gene Expression Regulation, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Time Factors.

Abstract

Nitrogen, an essential macronutrient for the growth and development of plants, affects above- ground biomass accumulation dramatically. Thus, it is very important to reveal the molecular mechanisms of how plants resist or adapt to low nitrogen availability. The NAC1(NAM, ATAF, CUC 1) gene, located in the upstream regulatory network, has been reported to resist low nitrogen by regulating expression of key downstream genes and thus root growth in (Populus tremula × alba).In this study, we detected the responses of miR164 and its target gene NAC1 under nitrate-starvation condition using the Betula luminifera somaclones G49-3 as material. The NAC1 gene which contains 1497 bp sequence, encodes 358 amino acids and contains a highly conserved NAM domain at N terminal was cloned by the RACE method. The NAC1 was then validated to be the target gene of miR164 via 5'-RACE, and the cleavage site was between the 10(th) and 11(th) base. The expression patterns of miR164 and its target gene NAC1 were further detected under nitrate-starvation condition through qRT-PCR analysis. The results showed that miR164 expression was repressed by nitrate-starvation at the beginning of the treatment (4 d) and then ascended. However, the expression pattern of miR164 in roots was different from that in shoots and leaves. Moreover, the expression levels of target gene NAC1 and miR164 were negatively correlated. The expression level of miR164 in root was increased while that of NAC1 was decreased under Re treatment, which indicated that miR164 and its target gene NAC1 play a regulatory role in response to low nitrate availability. The findings of our study may help elucidate the molecular mechanisms by which miR164 regulates target gene NAC1 at post-transcriptional level, and provide valuable information for further study of the regulatory roles of miR164-NAC1 under nitrate-starvation condition.

DOI: 10.16288/j.yczz.15-231
PubMed: 26907779

Affiliations:

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Le document en format XML

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<author><name sortKey="Wu, Jun" sort="Wu, Jun" uniqKey="Wu J" first="Jun" last="Wu">Jun Wu</name>
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<author><name sortKey="Zhang, Jun Hong" sort="Zhang, Jun Hong" uniqKey="Zhang J" first="Jun-Hong" last="Zhang">Jun-Hong Zhang</name>
</author>
<author><name sortKey="Huang, Meng Hui" sort="Huang, Meng Hui" uniqKey="Huang M" first="Meng-Hui" last="Huang">Meng-Hui Huang</name>
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<author><name sortKey="Zhu, Min Hui" sort="Zhu, Min Hui" uniqKey="Zhu M" first="Min-Hui" last="Zhu">Min-Hui Zhu</name>
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<author><name sortKey="Tong, Zai Kang" sort="Tong, Zai Kang" uniqKey="Tong Z" first="Zai-Kang" last="Tong">Zai-Kang Tong</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera].</title>
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<author><name sortKey="Zhang, Jun Hong" sort="Zhang, Jun Hong" uniqKey="Zhang J" first="Jun-Hong" last="Zhang">Jun-Hong Zhang</name>
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<term>Betula (genetics)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Gene Expression Profiling (methods)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>MicroRNAs (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Nitrates (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>RNA, Plant (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Time Factors (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term>
<term>Analyse de profil d'expression de gènes (méthodes)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Betula (génétique)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Facteurs temps (MeSH)</term>
<term>Nitrates (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>microARN (génétique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term>
<term>Plant Proteins</term>
<term>RNA, Plant</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betula</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term>
<term>Betula</term>
<term>Protéines végétales</term>
<term>microARN</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrates</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Nitrates</term>
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<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
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<term>Cloning, Molecular</term>
<term>Gene Expression Regulation, Plant</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Time Factors</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Facteurs temps</term>
<term>RT-PCR</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">Nitrogen, an essential macronutrient for the growth and development of plants, affects above- ground biomass accumulation dramatically. Thus, it is very important to reveal the molecular mechanisms of how plants resist or adapt to low nitrogen availability. The NAC1(NAM, ATAF, CUC 1) gene, located in the upstream regulatory network, has been reported to resist low nitrogen by regulating expression of key downstream genes and thus root growth in (Populus tremula × alba).In this study, we detected the responses of miR164 and its target gene NAC1 under nitrate-starvation condition using the Betula luminifera somaclones G49-3 as material. The NAC1 gene which contains 1497 bp sequence, encodes 358 amino acids and contains a highly conserved NAM domain at N terminal was cloned by the RACE method. The NAC1 was then validated to be the target gene of miR164 via 5'-RACE, and the cleavage site was between the 10(th) and 11(th) base. The expression patterns of miR164 and its target gene NAC1 were further detected under nitrate-starvation condition through qRT-PCR analysis. The results showed that miR164 expression was repressed by nitrate-starvation at the beginning of the treatment (4 d) and then ascended. However, the expression pattern of miR164 in roots was different from that in shoots and leaves. Moreover, the expression levels of target gene NAC1 and miR164 were negatively correlated. The expression level of miR164 in root was increased while that of NAC1 was decreased under Re treatment, which indicated that miR164 and its target gene NAC1 play a regulatory role in response to low nitrate availability. The findings of our study may help elucidate the molecular mechanisms by which miR164 regulates target gene NAC1 at post-transcriptional level, and provide valuable information for further study of the regulatory roles of miR164-NAC1 under nitrate-starvation condition.</div>
</front>
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<Abstract><AbstractText>Nitrogen, an essential macronutrient for the growth and development of plants, affects above- ground biomass accumulation dramatically. Thus, it is very important to reveal the molecular mechanisms of how plants resist or adapt to low nitrogen availability. The NAC1(NAM, ATAF, CUC 1) gene, located in the upstream regulatory network, has been reported to resist low nitrogen by regulating expression of key downstream genes and thus root growth in (Populus tremula × alba).In this study, we detected the responses of miR164 and its target gene NAC1 under nitrate-starvation condition using the Betula luminifera somaclones G49-3 as material. The NAC1 gene which contains 1497 bp sequence, encodes 358 amino acids and contains a highly conserved NAM domain at N terminal was cloned by the RACE method. The NAC1 was then validated to be the target gene of miR164 via 5'-RACE, and the cleavage site was between the 10(th) and 11(th) base. The expression patterns of miR164 and its target gene NAC1 were further detected under nitrate-starvation condition through qRT-PCR analysis. The results showed that miR164 expression was repressed by nitrate-starvation at the beginning of the treatment (4 d) and then ascended. However, the expression pattern of miR164 in roots was different from that in shoots and leaves. Moreover, the expression levels of target gene NAC1 and miR164 were negatively correlated. The expression level of miR164 in root was increased while that of NAC1 was decreased under Re treatment, which indicated that miR164 and its target gene NAC1 play a regulatory role in response to low nitrate availability. The findings of our study may help elucidate the molecular mechanisms by which miR164 regulates target gene NAC1 at post-transcriptional level, and provide valuable information for further study of the regulatory roles of miR164-NAC1 under nitrate-starvation condition.</AbstractText>
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<name sortKey="Wu, Jun" sort="Wu, Jun" uniqKey="Wu J" first="Jun" last="Wu">Jun Wu</name>
<name sortKey="Zhang, Jun Hong" sort="Zhang, Jun Hong" uniqKey="Zhang J" first="Jun-Hong" last="Zhang">Jun-Hong Zhang</name>
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[Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera].

[Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki